Preparation and Quantitative Analysis of Ursolic Acid Separation from Herba Hedyotidis Corymbosae

Objective: To separate and extract the Ursolic acid from Hedyotis corymbosa Lam by adopting the method of ethanol extracting and agglutination and to determine the content of ursolic acid . Method: Using spectrum to identify the structure of ursolic acid by IR, MS,1HNMR 13CNMR. Adetermination method of ursolic acid by reversed-phase high performance liquid chromtograph was established. The chromatographic column, SymmetryShield RP18 (3.9×150 mm,5 μm), methanol-water (88:12, V/V) mobile phase with 1.0 mL/min flow rate, the detected wavelength (210 nm), and the column temperature(25 ℃) were adopted. Result: The compound is recognized to be ursolic acid. The calibration curve of ursolic acid by HPLC was linear under the content of 4.5～22.5 μg, the correlation coefficient was over 0.9998. The content is 99.82% and the gain rate is 3.65‰. Conclusion: The preparation method is practical and feasible. The purity of ursolic acid is high and the safety is good. The analytical method is simple, accurate and has good repeatability and wider linear range. It can be adapted to evaluate the quality of ursolic acid.