2007•Anhui nongye kexueRequires access
Separation and HPLC Analysis of Ursolic Acid in Hedyotidis Corymbosae Lam
Zhu Zhiyong
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Abstract
To develop a method for extracting and analysis of ursolic acid in Hedyotidis Corymbosae Lam.the method of ethanol extracting and agglutination separating were used to extract ursolic acid from Hedyotidis Corymbosae Lam and the structure of ursolic acid was identified by IR,MS,NMR.HPLC was carried out on the chromatographic column of SymmetryShield RP18(3.9 mm×150 mm,5 μm)at 25 ℃,using methanol-water(88∶12,V/V)as mobile phase with a flow of 1.0 ml/min,and detection wavelength was set at 210 nm.There was a good relationship between the peak area and the sample content injected at the range of 3.9～19.6 μg(r = 0.999 9).The content was 99.58% by HPLC and the gain rate was 3.04‰.The method was simple,accurate and had good repeatability and wider linear range,and can be used to evaluate the quality of ursolic acid.