Preparation and HPLC Analysis of Ursolic Acid Separation fromHerba Hedyotidis Corymbosae

This paper explores adopting the method of ethanol extracting and agglutination separating to extract ursolic acid from Herba Hedyotidis Corymbosae and using spectrum to identify its'structure by IR, MS, NMR.A determination method of ursolic acid in Herba Hedyotidis Corymbosae by reversed-phase high performance liquid chromtograph was established. The chromatographic column, SymmetryShield RP18 (3.9 mm×150 mm,5 μm), methanol-water (88∶12, V/V) mobile phase with 1.0 mL/min flow rate, the detected wavelength (210 nm), and the column temperature(25 ℃) were adopted. The calibration curve of ursolic acid was linear under the content of 4.5～22.5 μg, the correlation coefficient was over 0.999 8. The content is 99.82% by HPLC and the gain rate is 3.65‰. The method is simple,accurate and has good repeatability and wider linear range. The method can be used to evaluate the quality of ursolic acid.