Study on Determination of Ursolic Acid in Fructus gardeniae by HPLC-PAD

A determination method of ursolic acid in Fructus qardeniae by reversed-phase high performance liquid chromatograph was established and at the time the purity of ursolic acid peak was detected by means of Photodiode Array Detector(PAD).The chromatographic column,Nucleosil C18柱(4.6 mm×250 mm,5 μm),water-methanol-acetic acid(85:15,V/V) mobile phase with 0.8 mL·min~(-1) flow rate,the detected wavelength(205 nm),and the column temperature(25 ℃) were adopted.The calibration curve of ursolic acid was linear under the content of 0.1696 ~1.5264 μg,the correlation coefficient was over 0.9999, the detected limit was 0.3 mgL~(-1)(3σ).The average recovery of ursolic acid was 98.0 %.The method is simple,accurate,reproducible and has wider linear range.The method can be adapted to determine ursolic acid in Fructus gardeniae.