Effect of Basic Fibroblast Growth Factor(BFGF) on Proliferation and Dose-effect Relationship of Tendon Cells in vitro

【Objective】To study effects of BFGF of different concentration on proliferation and dose-effect relationship of tendon cells cultured in vitro and estimate the optimal BFGF concentration to promote proliferation routinely.【Methods】The groups of different BFGF concentrations were stained by MTT methods and their optical density(OD) average values were detected by enzyme linked immunodetection meter.【Results】By contrast to OD mean of control group: 0.5、1.0、2.0、5.0 、30、40 、50ng/mL BFGF group had significant difference(P0.05);0.5 and 20ng/mL BFGF group had no significant difference(P0.05).When BFGF concentration varying from 0ng/mL to 5.0 ng/mL,OD mean of every group increased gradually,while from 5.0 ng/mL to 50 ng/mL,it decreased step by step.【Conclusion】BFGF of could promote proliferation of tendon cells;0.5ng/mL and 20ng/mL BFGF has no significant effect on proliferation of tendon cells;30ng/mL、40ng/mL and 50ng/mL BFGF could inhibit proliferation of tendon cells.And 5.0ng/mL is the optimal BFGF concentration to promote proliferation.