Effect of base fibroblast growth factor on proliferation of rat pancreatic stem cells

Objective To investigate the effect of basic fibroblast growth factor(bFGF) on proliferation of pancreatic stem cell(PSC) of SD rat.Methods PSCs were cultured in vitro after being purified.CK-19 and Pdx-1 expression were detected by immunohistochemistry to identify the proliferative characteristics of PSC.Different concentrations of bFGF were used to study the effect on grow curve of PSCs.Results The purified primary-cultured PSCs were obtained after repeated adherence.Immunohistochemistry results showed positive expression of CK-19,and negative expression of Pdx-1 in PSCs.The PSC number of control group,being cultured with no bFGF and of the experimental groups,being cultured with(5 ng/ml),(10 ng/ml) and(20 ng/ml) bFGF was 3.29×10~5/ml,3.47×10~5/ml,3.83×10~5/ml and 3.83×10~5/ml respectively.The PSC numbers of group cultured with(10 ng/ml) and(20 ng/ml) bFGF were significantly higher than that of(5 ng/ml) bFGF group(P0.01).The cell numbers of all three bFGF groups were significantly higher than that of control group(no bFGF)(P0.01).Conclusion All three different bFGF concentrations((5 ng/ml),(10 ng/ml) and(20 ng/ml)) can significantly stimulate the proliferation of PSC.