Induction and Propagation of Cymbidium hybridum Protocorms

The study aimed to discuss the optimum formula for inducing and propagating Cymbidium hybridum protocorm.With the aseptic seedling of Cymbidium hybridum as explants,the MS and 1/2MS were taken as the basic medium adding different concentration of 6-BA,2,4-D and NAA,the formula of Cymbidium hybridum protocorm induction and propagation were studied.The result shows that the protocorm induction rate is the highest at 77.78% in the medium of 1/2MS + 6-BA 8.0 mg/L + 2,4-D 0.2 mg/L + agar 10g/L + sucrose 15g/L + AC 1.5g/L(final pH=5.8),and the protocorm propagation effect is the best in MS + 6-BA 6.0mg/L + NAA 0.2mg/L + agar 10g/L+ sucrose 10g/L + AC 1.5g/L + Banana puree 100g/L(final PH=5.8).There are significant effect of lower concentration of 2,4-D(0.2 mg/L)and higher concentration of 6-BA(8.0mg/L) on the induction of Cymbidium hybridum protocorm.