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2010•Guizhou nongye kexueRequires access

Induction and propagation of Hybridum cymbidium protocorms.

S. F. Lisa, Yi Yin, Ximin Zhang, Yubin Zhang

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Abstract

The aseptic propagation system of Hybridum cymbidium protocorms was established by the tissue culture of its stem apex and section.The results showed that the optimum induction media for bud,and protocorm were 1/2MS+0.2mg/L NAA+1.0 mg/L 6-BA +100 mg/mL VC and 1/2MS+0.2 mg/L NAA+2.5 mg/L 6-BA +100 mg/mL VC respectively,the optimum multiplication medium for protocorm induction was 1/2MS+0.3 mg/L NAA+3.0 mg/L 6-BA +100mg/mL VC,and the suitable explant for protocorm multiplication of Hybridum cymbidium was protocorm.

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What this paper is about

The aseptic propagation system of Hybridum cymbidium protocorms was established by the tissue culture of its stem apex and section.The results showed that the optimum induction media for bud,and protocorm were 1/2MS+0.2mg/L NAA+1.0 mg/L 6-BA +100 mg/mL VC and 1/2MS+0.2 mg/L NAA+2.5 mg/L 6-BA +100 mg/mL VC respectively,the optimum multiplication medium for protocorm induction was 1/2MS+0.3 mg/L NAA+3.0 mg/L 6-BA +100mg/mL VC,and the suitable explant for protocorm multiplication of Hybridum cymbidium was protocorm.

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Available abstract

The aseptic propagation system of Hybridum cymbidium protocorms was established by the tissue culture of its stem apex and section.The results showed that the optimum induction media for bud,and protocorm were 1/2MS+0.2mg/L NAA+1.0 mg/L 6-BA +100 mg/mL VC and 1/2MS+0.2 mg/L NAA+2.5 mg/L 6-BA +100 mg/mL VC respectively,the optimum multiplication medium for protocorm induction was 1/2MS+0.3 mg/L NAA+3.0 mg/L 6-BA +100mg/mL VC,and the suitable explant for protocorm multiplication of Hybridum cymbidium was protocorm.

Key concepts: Cymbidium, Explant culture, Tissue culture, Botany, Horticulture, Aseptic processing, Biology, Apex (geometry)

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