Research on High-regeneration Factors on Multiple-buds of Cymbidium hybridum

For the study of influencing high frequency on multiple factors of Cymbidium hybridum,by asepsis protocorm used of protocorm for growing seedlings of induction,regeneration and rooting breeding research. Results show that in 1 /2 MS + AC 1. 0 g / L + NAA 0. 4 mg / L + 6- BA 0. 5 mg / L + sucrose 25 mg / L + agar cultivation of 10 g / L,the induction rate is as high as 75. 1%,cluster bud seedling growth; In 1 /2 MS + agar 8 g / L + 1. 0 g / L + 6- BA 2. 5 mg / L + NAA 0. 5 mg / L + sucrose 18 g /L of medium induced the most number of multiple shoot clumps,up to 5. 13 times; 6- BA and NAA on induction of proliferation was significant; The bulb is an effective way to induce large fourth multiple shoot clumps investigate.