Study on Tissue Culture and Regeneration System of Jujube Variety Luzao 1

The growing shoots of jujube variety Luzao 1 were used to study the bud induction and rapid propagation of test tube plantlets.The results showed that the latent bud of shoot stem segments could be induced to break on the induction medium,which was MS supplemented with 1 mg/L BA,0.2 mg/L IBA and 3% sucrose,and the bud break rate was 54.8%.On the same induction medium,adventitious bud could be induced to regenerate from the distal cut surface of stem section not touch medium,and the regeneration rate was 23.8%.The propagation of adventitious bud and latent bud was better on the medium of MS+2 mg/L BA+0.4 mg/L IAA+3%sucrose+6 g/L agar.The rooting rate of test tube plantlets was over 70% on the medium of 1/4MS+0.5 mg/L IBA+2%sucrose+6 g/L agar.