Induction of adventitious buds from in vitro leaves in Chinese jujube

Efficient induction of adventitious buds from in vitro Chinese jujube leaves was achieved using Ziziphus jujuba Mill.cv.'Yueguangzao','Dongzao' and 'Muzao'.Callus occurred in 100% of leaves from three cultivars cultured in medium MS+maltose 20 g/L+agar 5 g/L+TDZ 1.0 mg/L+IBA 0.1 mg/L+AgNO3 1.0 mg/L.The adventitious buds rate obviously changed in differentiation mediums separately supplemented with GA3,BA,TDZ.The medium MS+ sugar 30 g/L+ agar 5 g/L+ IBA 0.2 mg/L+6-BA 0.8-1.2 mg/L was appropriate for adventitious bud induction in 'Yueguangzao',in which the adventitious bud rate and the average number of buds in per leaf block respectively reached 93.3% and 3.53.Especially,no vitrification appeared in the regenerated buds.The adventitious bud rate in both 'Dongzao' and 'Yueguangzao' was higher than 93% in medium MS+sugar 30 g/L+agar 5 g/L+IBA 0.2 mg/L+6-BA 0.8 mg/L.In contrast,it was significantly low and only 66.63% in 'Muzao'.In addition,AS(Acetosyringone) 100 mg/L significantly accelerated adventitious bud formation in 'Dongzao' and the average number of buds in per piece of leaf was as high as 7.30.