2004•Journal of Jishou UniversityRequires access
Study on Fast Propagation of Juj ube C. V. Jidanzao by Tissue Culture
WU Cheng-hou, He YeHua, Bixia Xie, Hu Fangming
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Abstract
The induction of adventitious buds from stem segments and the forming of plantlets in vitro were studied in Jidanzao jujube .The results showed that adventitious buds could be induced from stem segments on the culture medium of MS+ ZT 1.75 mg·L~(-1)+ KT 2.0 mg·L~(-1).The addition of low concentrations of NAA to the medium made higher induction rates of adventitious buds and 4.2 times,the optimal proliferation number,was gotten on the medium of MS + ZT 1.75 mg·L~(-1)+ KT 2.0 mg·L~(-1)+NAA 0.05 mg·L~(-1).When the shoots formed in vitro got length of 3～4 cm,they were used for the induction of adventitious roots.The optimal medium for this purpose was 1/2 MS+IBA 0.8 mg·L~(-1) and the forming rate of plantlets was over 95.0%.In addition,the sterilization of explants and the transplant of plantlets were also studied.