Tissue Culture and Establishment of Regeneration System of Kashihare Grape

In this study,an efficient system of shoot regeneration has been established by use of leaves of Kashihare grape.The results showed that the best auxiliary bud germination medium is MS+6-BA 1.0 mg/L+NAA 0.01 mg/L,with the germination rate of 100%.The best subculture medium is MS+KT 0.1 mg/L+IBA 0.4 mg/L.The auxiliary bud germination and the root growth took place at almost the same time in subculture medium,and root rate was up to 100%.The type of the basic medium,the part of explants,the influence of plant growth regulators were investigated in inducing adventitious bud.The results indicated that the highest regenerating rate of adventitious buds were obtained when leaf explants were incubated on MS+6-BA 2 mg/L+IBA 0.2 mg/L + Sucrose 25 g/L.The medium MS+KT 0.1 mg/L+IBA 0.4 mg/L+Sucrose 20 g/L was the best for roots inducing,with an average inducing rate of 92%.