Plant Regeneration and Callus Induction of Three Lines of Peppers via Cotyledon Culture

Taking male sterile line,maintainer line and restorer line of peppers cotyledon as explant,the factors effect of 6-BA,IAA concentration and AgNO3 on the in vitro regeneration culture of pepper cotyledon.The results showed that three lines of peppers had differences on callus and adventitious bud induction not merely the best medium of callus and adventitious bud induction but also the number of bud.The bud of R-1 easily outnumber A-1 and B-1;AgNO3 didn't had an obvious effect on callus induction,but 3 mg/L AgNO3 could enhance the rate of adventitious bud differentiation;The higher 6-BA/IAA ratio of ten was conductive to the differentiation of the explants of cotyledons than it was four;1/2MS+IAA 0.2 mg/L+NAA 0.1 mg/L was the optimal medium of rooting by regenerated shoots.