Effect of Hormone Combinations on Callus Induction and Adventitious Bud Differentiation of Pepper Leaves

Taking the section 9～11blade male sterile pepper ‘ 071 ’ as explants,the effects of different concentrations of hormone combinations on callus induction and adventitious bud differentiation of pepper leaves were studied.The results showed that the callus induction effect of the use of 6-BA and NAA was good,but could not promote adventitious bud differentiation occurred.On the medium of 6-BA 3.0mg / L+NAA 0.1mg / L,the callus had the fastest induction speed,with callus induction rate 100%.The concentration of NAA in the 0.05,0.1mg / L,6-BA concentration in 0.2～5.0mg / L,the higher the concentration of 6-BA,the callus was prone to browning;and with the use of 6-BA and IAA,the callus differentiation rate was about 50%,and could promote the occurrence of adventitious bud differentiation.With 6-BA 5.0mg / L+IAA 0.5mg / L ratio,bud induction rate of leaf blade was higher for 35.29%.The concentration of TDZ was 0.05,0.1mg / L,used alone or in conjunction with the NAA,IAA,all without exception bud emergence.