Isolation of the cDNA fragment of watermelon genic male sterility related genes using DDRT-PCR

【Objective】 The research was done in order to study the molecular mechanism of genic male sterility in watermelon.【Method】 mRNA differential display was used to analyze the differential expression of genes between male sterile flower buds and male fertile flower buds in male sterile watermelon Se18.【Result】 Through identification of repeated tests,the differential fragments T12C/B0315S-359 were obtained in male fertile flower buds;two differential fragments were obtained in male sterile flower buds:T12C/B0315F-175 and T12G/B0318F-260.The T12C/B0315S-359 fragment was 82% homologous with a code sequence of an unknown protein which involved in vascular development of quaking aspen.The result of GenBank BLAST showed that the T12C/B0315F-175 fragment had 98% nucleotide similarity to the ycf5 gene encoding cytochrome C assembly protein in cucurbitaceous plants.The homology research indicated that T12G/B0318F-260 fragment was of 84% of homology with code sequence of mRNA of thioredoxin peroxidase in plants.【Conclusion】 After sequence research,the three differential fragments were related to genic male sterility in watermelon Se18.