Analysis of Photoperiod-temperature Sensitive Male Sterility Related Genes in Wheat with DDRT-PCR

The wheat BS210, a line identified as photoperiod-temperature sensitive genic male sterility, was grown in different ecological environment (Shunyi district in Beijing for fertile, and Fuyang in Anhui province for sterile ). The total RNAs were isolated from the young fruiting spikes of plants in different developing period (stamens/pistil differentiation, anther chamber formation, pollen mother cell formation, tetrad phase, and mono-nucleate phase), and the differential expression of the genes were analyzed with mRNA different display technique. 20 differential expression DNA bands were identified with reverse Northern hybridization, and the positive clones were sequenced. The 4 differential expression genes were selected as candidate genes through the BLAST research in GenBank, and therefore the 5′RACEs were conducted. The further homology research indicated that candidate genes A3, B2 and A2 were highly homological to Oryza sativa DNA repair-recombination protein (rad50), Triticum aestivum receptor-like kinase with LRR repeats and Zea mays lethal leaf-spot 1 with 89%,89% and 88% identity, respectively, while the candidate gene B1 was a novel gene. Interestingly, compared with themselves between the sterile and fertile conditions, high homology was observed in the 5′ region of mRNAs of B1 and A3, while the lengths of 3′ region of mRNAs are different. This study provided some useful information for further understanding the mechanism of photoperiod-temperature sensitive genic male sterility of wheat.