DIFFERENTIAL EXPRESSION ANALYSIS OF GENIC MALE STERILITY BY cDNA-AFLP IN MAIZE

The differential expression of male sterility induced by space flight with male fertility was studied using cDNA-AFLP technology.Total RNA was isolated from anther of male sterility and male fertility.Nine differential expression cDNA fragments were obtained with 16 primer combinations.The differential cDNA fragments were eluted,cloned and sequenced.Then half-quantitative RT-PCR was used to study the differential expressions of 4 development stages between sterility and fertility.Sequencing analysis shown 2 fragments from male sterility might be novel genes.Four fragments from male fertility were homology as chalcone and stilbene synthases,putative acyl CoA dehydrogenase,putative protein kinases and putative glycine decarboxylase.All these proteins might participate in the energy metabolisms,substance metabolisms or signal transmission of gametophyte development.Half-quantitative RT-PCR shown Z3 had a high expression in the early stage of pollen development,Z8 took on increasing expression during the middle period of pollen development.These results just met the demand of more energy and more substance during the pollen development.