Effects of the Formula of invigorating spleen to resolve dampness and Nourishing stomach Yin on Endothelin-1 and Nitric oxide in the rats of type 2 diabetes

Objective To observe the effects of the Formula of invigorating spleen to resolve dampness and Nourishing stomach Yin on Endothelin- 1 and Nitric oxide in diabetic rats. Methods The model of type 2 diabetes was established by intraperitoneal injection of Streptozotocin( STZ) at a dose of 30mg / kg and a high- carbohydrate / high- fat diet. Experimental rats were randomly divided into six groups: normal group,model group,Benazepril Hydrochloride group,combination group,invigorating spleen to resolve dampness group,Nourishing stomach Yin group and they were treated respectively. Eight weeks later,ET- 1,NO, NOS contents were determined through ELISA and nitrate reductase method respectively. Results( 1) The contents of creatinine and urea nitrogen in serum of rats were without significant difference( P 0. 05). Renal coefficients in model group was markedly elevated( P 0. 05),the intervention groups compared with model group were significant decreased( P 0. 05).( 2) NO, NOS,NO / ET- 1 ratio in the model groups compared with the normal group were markedly elevated; after intervention therapy, combination group and Nourishing stomach Yin group were declined significantly( P 0. 05). Conclusion It is possible that invigorating spleen to resolve dampness and Nourishing stomach Yin Decoction delay the occurrence and development of diabetic nephropathy by reducing NO content and NO / ET- 1.