Effect of Method of Nourishing Yin and Tonifying Liver and Kidney on Contractive Pressure,ET-1 and ANG II in Renal Hypertension Rats with Syndrome of Yin Deficiency Leading to Yang Hyperactivity

Objective:To investigate the mechanism of method of nourishing yin and tonifying liver and kidney on renal hypertension rats with syndrome of yin deficiency leading to yang hyperactivity.Methods:78 selected healthy male SD rats aged 2 months were randomly divided into sham operation group(13 rats) and operation group(65 rats).In operation group the models of renal hypertension were made up by ligation of the left renal artery,and among them the hypertensive rats were screened by method of tail cuff to measure blood pressure.Then Aconite Decoction was given to them for 4 weeks,and every one day they were provoked to fight in order to prepare the models with syndrome of yin deficiency leading to yang hyperactivity.The successful models of RHR with syndrome of yin deficiency leading to yang hyperactivity were screened and randomly divided into model group,captopril group,group of nourishing lung yin,group of tonifying liver and kidney,and group of nourishing yin and tonifying liver and kidney(synthetic group).The medication groups were given to intragastric administration at eight in the morning,and sham operation group and model group were given to equal volume of normal saline.The course of treatment included 4 weeks.After 24h in the last administration the blood pressure was measured,and 8mL blood in abdominal aorta was taken and prepared into serum and plasma.Then the concentrations of ET-1 and angiotensin Ⅱ in serum were determined by ELISA method.Results:On blood pressure,①There were not significant difference on the basic blood pressure between sham operation group and other groups(including model group,captopril group,group of nourishing lung yin,group of tonifying liver and kidney,and synthetic group(P 0.05).②The second week after operation,the blood pressure increased markedly in each group compared with sham operation group(P 0.05).③After 4 weeks' treatment the blood pressure in captopril group,group of tonifying liver and kidney and synthetic group decreased obviously compared with that in model group(P 0.05),but group of nourishing lung yin did not show statistical significance(P 0.05).The blood pressure also decreased in aptopril group,group of tonifying liver and kidney and synthetic group markedly compared with group of nourishing lung yin(P 0.05).And compared with captopril group the blood pressure cut down remarkably in synthetic group(P 0.05),but in group of tonifying liver and kidney no significant difference show up(P 0.05).On ET-1,ANG Ⅱ and NO,①ET-1 and ANG Ⅱ increased obviously in model group compared with sham operation group(P0.05).②ET-1 and ANG Ⅱ decreased markedly in captopril group,group of tonifying liver and kidney and synthetic group compared with model group(P 0.05).They were cut down too in group of nourishing lung yin,but no significant difference showed up(P 0.05).?There were significant differences between captopril group,group of tonifying liver and kidney and synthetic group and group of group of nourishing lung yin(P 0.05).④ET-1 and ANG Ⅱ decreased in synthetic group more than in captopril group(P 0.05),but in group of tonifying liver and kidney there was no significant differences(P 0.05).Conclusion;The mechanism of nourishing yin and tonifying liver and kidney on renal hypertension rats with syndrome of yin deficiency leading to yang hyperactivity lies in reducing the formation of vasoconstrictor ET-1,weakening myocardial contractility induced by the ET as well as increased cardiac output and vascular wall thickening,reducing the formation of ANGⅡ,against vascular contraction caused by ANG Ⅱ as well as vessel wall thickening and compliance depressing.