Cloning of Bovine Adiponectin Whole Gene cDNA

[Objective] The aim was to clone the whole coding sequence of bovine adiponectin gene in vitro.[Method] The whole sequence of cDNA from bovine adiponectin gene was amplified from the total RNA of adult bovine tail fat by RT-PCR and then it was inserted into PMD18-T expressive vector.The positive clones were screened,then recombinant plasmid was extracted.After identified with restriction endonuclease and identified with recombinant plasmid by PCR,the positive clones were sequenced.[Result] The target band of 671 bp was amplified from the total RNA of bovine tail fat.The cDNA sequence was 100% identical to that of bovine adiponectin gene in Genbank and the amino acid homology reached 100% too,which showed that it was target gene.[Conclusion] The research provided the experimental basis for the further study on the function of bovine adiponectin gene and the relationship between the expression of bovine adiponectin gene and the fat metabolism of fattening cattle.