1997•Journal of Veterinary Medical ScienceOpen access

Molecular Cloning of Bovine CD14 gene.

Akemi Ikeda, Masuhiro Takata, Takahide Taniguchi, Kenji Sekikawa

Abstract

Genomic DNA encoding bovine CD14 was isolated from a bovine (Holstein) genomic library. Utilizing PCR fragment of mouse CD14 gene as a probe, we screened 9 x 10(5) plaques and obtained 3 clones containing the bovine CD14 gene. DNA sequencing showed that bovine CD14 gene encodes 373 amino acids, and the coding sequence was separated by a 90 nt intron. The identity of the deduced amino acid sequence of bovine CD14 was 61-73% to those of mouse, rabbit and human. Northern blot analysis revealed that CD14 mRNA (1.5 kb) was expressed in the lung. Expression of CD14 mRNA was stimulated about 2-fold in bovine peripheral blood macrophage activated with LPS in vitro.

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Key concepts: Molecular biology, genomic DNA, Gene, Biology, Coding region, Northern blot, CD14, Sequence analysis