2005•Unpublished venueRequires access

Cloning and Sequencing of cDNA of Human Adiponectin

Jie Zhang

Abstract

Objective: To clone cDNA of human adiponectin for nucleotide sequencing. Methods: The total RNA of human adipose tissue was extracted.The cDNA complete coding region of human adiponectin was amplified by RT-PCR.The product was cloned into plasmid pMD18-Tby TA-clone.The recombinant plasmid was verified by sequencing. Results: The clone of cDNA of human adiponectin was successfully constructed.The recombinant plasmid(pMD18-T/ADPN)was verified to share 100%homologies with that of genebank by sequencing. Conclusion: The cloning of human adiponectin cDNA is adapted to further expression.

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Key concepts: Complementary DNA, Cloning (programming), clone (Java method), Recombinant DNA, Adiponectin, Biology, Plasmid, Molecular biology