Surveillance of antimicrobial resistance in clinical isolates of Klebsiella pneumoniae

Objective To investigate the resistance in clinical isolates of Klebsiella pneumoniae and offer experimental data for reasonable antimicrobial agents.Methods The microbial sensitivity tests of 392 clinical isolates of Klebsiella pneumoniae were tested by micro-dilution method.Extended spectrum beta-lactamases (ESBLs) were screened by micro-dilution method and confirmed by CAZ+CD2 and CTX+CD3.Results Resistant rates over 30% added up to 11 among the 18 tested antimicrobial agents.Resistant rates of Klebsiella pneumoniae against ampicillin,ampicillin-sulbactam,cephalothin,piperacillin,trimethoprim-sulfamethoxazole,cefacidal,respectively,accounted for more than 50%.Resistance rates of four antimicrobial agents were lower than 10 percentage.They were ceftriaxone (7.7%),cefetaxime (7.4%),aztreonam (6.9%),imipenem (3.1%).Incidences of ESBLs producing strains were from 32.9% to 45.8%.The average was 39.8%.Resistant rates of ESBLs producing strains were much higher than ESBLs nonproducing strains (P0.05).Conclusions Resistant rates of clinical isolates of Klebsiella pneumoniae were rather high and multidrug-resistance,especially in ESBLs producing strains.We should give more attention to the surveillance of antimicrobial resistance in clinical Klebsiella pneumoniae and prevent transmission and epidemic of those resistant strains.