Analysis of the antimicrobial resistance of 53 strains of Klebsiella pneumoniae

Objective To study the antimicrobial resistance of Klebsiella pneumoniae and the isolation rate of extended spectrum β lactamases (ESBLs) producing strains in a hospital. Methods Antimicrobial resistance of the identified strains of Klebsiella pneumoniae was detected by disk agar diffusion test, and extended spectrum β lactamases (ESBLs) producing strains were detected by double disc synergy test. Results Fifty three strains of Klebsiella pneumoniae were isolated, 26 ( 49.06% ) of which were ESBLs producing strains. The resistant rate of Klebsiella pneumoniae to the common antimicrobials was as follows: 98.11% (ampicillin ); 73.58% (piperacillin); 69.81% (tetracycline); 67.92% (chloramphenicol); 67.92% (sulphamethoxazole/trimethoprim); 60.38% (cephazolin); 54.72% (gentamycin); 52.83% (cefuroxime); 49.06% (augmentin); 49.06% (cefotaxine); 49.06% (nalidixic), 41.51% (amikacin); 37.74% (aztreonam); 32.08% (ciprofloxacin); 10.00% (ceftazidime); 0.00% (imipenem). The antimicrobial resistant rates of majority of ESBLs producing strains were above 60%, which were significantly different from the non EBSLs producing strains (P 0.005 ) with the exception of ampicillin, imipenem, tetracycline and chloramphenicol. Conclusion Imipenem is the first choice antimicrobial agent to treat the infection with EBSLs producing strains of Klebsiella pneumoniae, measures should be taken to prevent the prevalence of nosocomial infection with Klebsiella pneumoniae and the emergence of ESBLs producing strains.