Optimization of Extraction Process of Compound Niuhuang Capsules

Objective: To optimize extraction process of compound Niuhuang capsules. Method: UV was employed to determine the content of total saponins with detection wavelength at 560 nm and 545 nm. Taking quality of total saponins as index,orthogonal test was adopted to optimize water extraction process with the amount of water,soaking time,extration time and times as factors; the same method was used to investigate effects of ethanol concentration,ethanol amount,extraction time and times on alcohol extraction process. Result: The best water extraction process was: soaked 0. 5 h with 10 times the amount of water,decocted two hours for three times;optimum alcohol extraction process was as following: decocted thrice with 8 times the amount of 70% ethanol for 2h each time. Conclusion: These extraction process is stable and feasible,this study can provide an experimental basis for clinical application of compound Niuhuang capsules.