Optimization of extraction process for liposoluble components from Danxinshu capsules by orthogonal test

Objective To select the optimal extraction process for liposoluble components from Danxinshu capsules.Methods L9(34) orthogonal test was conducted to evaluate the effects of ethanol concentration,ethanol consumption,extraction duration,and extraction time on the extraction by using the content of four kinds of sanchinosides and the content of the total flavonoids in axillary choerospondias fruit that were included in the recipe of Danxinshu capsules.HPLC and ultraviolet spectrophotometry were used in the assay of cumarin.Results The optimal condition for the extraction process: extract tow times with 70% ethanol,one hour per time;ethanol volume: ten times of that of the medicinal substances.Conclusion The process was rational and feasible,which could be used as the reference for mass production.