The development of chicken bone marrow-derived dendritic cells inducing with cytokines in vitro

To explore the methods of inducing dendritic cells(DCs) from chicken bone marrow cells in vitro,chicken bone marrow cells were taken from 10-day-old chicken and cultured in the presence of recombinant human granulocyte-macrophage factor(rhGM-CSF) and rhIL-4.The results demonstrated that the cells showed typical morphologic and phenotypic characteristics of DCs after 8 day's cultivation and expressed higher level of CD11c as the surface marker of DCs detected by FACS staining with PE-conjugated anti-CD11c antibody.The feasibility for inducing chicken bone marrow cells with GM-CSF and IL-4 to generate DCs was demonstrated in this study.Thus,GM-CSF and IL-4 are required for the differentiation of the bone marrow-derived DCs in chicken.