Culture and Identify Dendritic Cells Originated From Bone Marrow and Spleen of Mouse

Objective To establish a method of cultivation and purification of dendritic cells(DCs) from mouse bone marrow and spleen in vitro and provide experimental material and establish basis for the study of immunological tolerance.Methods Extracted the ICR mice spleen lymphocytes and bone marrow cells under sterile conditions,and cultured DCs in co-induced of recombinant granulocyte-macrophage colony-stimulating factor(GM-CSF) and interleukin4(IL-4).Then,the changes of DC morphology were observed under light microscopy and CD80,CD86 expression levels were detected with flow cytometry.Results bone marrow cells appeared irregular and formed dendritic processes after 3 days cultured with stimulating factor in vitro which showed the typical morphology of dendritic cells,and costimulatory molecules(CD80,CD86) were low expression on the cellular suface.Conclusion Compared with the spleen cells,bone marrow cells are not only rich in DC precursor cells but also induced into DCs with a short time.