Isolation and Identification of GXBB Strain of Porcine Pseudorabies Virus and Sequence Analysis of gE Gene

One virus was isolated from brain and visceral organs of diseased piglets from a pig farm in Bobai of Yulin of Guangxi.The virus that infected rabbits caused typical pseudorabies clinical symptom,induced typical cytopathogenic effect(CPE) after inoculated in PK-15 cell.And virus TCID50 was 10-7.22/0.1 ml.A specific band about 947 bp was obtained by PCR amplification with a pair of primers designed according to a reference.The PCR product was cloned,sequenced and compared with MinA strain,Ea strain,SH strain,LA strain,GXB strain,GXW strain,the result displayed 98.7% to 99.4% nucleotide sequence homology and 98.1% to 99.1% amino acid sequence homology,so it was comfirmed as pseudorabies virus and it was named GXBB strain.Phylogenetic analysis revealed that the PRV GXBB strain and other PRV strains were closely related and there were few mutations about the epidemic PRV strains in Guangxi at present.The study provides the scientific theoretical basis for prevention and eradication of PRV.