Isolation and identification of porcine pseudorabies virus(PRV)C strain

One porcine pseudorabies virus was isolated from epidemiological investigation,which was named PRV C strain,then the specificity of the strain was studied.The special prime during the region gl and gE was designed to check the strain by PCR,it showed that objective band of Min A strain lied at about 5 000 bp,but the strain lied at about 1 500 bp gene sequencing showed that die sequencing was 1 538 bp.The gene deletion of the strain was during 989—4 468,about 3 570 bp.The deletion consisted of the main virulence(gE),the mutations happened at the site of 873,4 665,4 666,4 950.The homology of the strain and Bartha-K61 was during95.0%—96.0%,so it was different from the Bartha-K61.The neutralization test was done on neutralization of the virus antibody by the 14 strains PRV isolated from some,provinces in China and Min A strain.The neutralization indexes were 708—6 761,the strain could neutralize all the strains PRV,which indicated that it could be the candidate vaccine strain.