Sequence determination and comparison of gE gene fragment of Pseudorabies virus SH strain

The PCR assay of Pseudorabies Virus(PRV) was established,and the amplified fragment of gE gene of PRV SH strain was sequenced and analyzed.Two pairs of PCR primers were designed according to the gE and gB genes of PRV.The PRV SH strain and Herpes simplex virus strain I were used as a template and the PCR product was cloned into pMD18-T vector.The recombinant plasmid was sequenced and compared with the Fa strain,and their homology was 98%. In comparison with the gE gene sequence of PRV recorded in the Genebank,the homology was above 97%.The sequence of gE gene is comparatively conserved and has a rather high value in vaccine application and molecular diagnosis.