Isolation and Identification of GDSH Strain of Pseudorabies Virus and Sequence Analysis of gE Gene

Isolates were got from brains and visceral organ samplesf of piglets. The typical cytopathic effect( CPE) could be seen when the isolates were cultured on the PK- 15 cells and its TCID50 was 10-8/0.1mL. Typical clinical signs could be observed when the pigs and mice were injected with the isolate. The isolate was named as GDSH strain of Pseudorabies virus. According to the genomic sequence of PRV in GenBank, PCR primer was designed. PCR method was establlshed to differentiate wild PRV and vaccine strains. And then the PCR product was cloned and sequenced.The sequence data of PRV isolate was analysed using the DNAStar software. The results demonstrat- ed that the necleotide identity of PRV gE- gene was 98.3%- 99.9% comparing with other PRV isolates published in GenBank, and with 99.9% necleotide identity compared with PRV strain Ea.