Value of time-resolved fluorescence immunoassay in detection of HBsAb

Objective To discuss the advantages and clinical value of detection of hepatitis B surface antibody(HBsAb)by time-resolved fluorescence immunoassay(TrFIA).Methods HBsAb was detected in 262 serum samples by ELISA and TrFIA,using EXCEL 2003 to finish statistics and data analysis,assessing the consistency of detection results with Kappa and u test.Results The positive rate of HBsAb was 53.8%(141/262)by ELISA and 56.9%(149/262)by TrFIA.The consistency rate was 90.8%,K-value was 0.815 with Kappa test.Consistency was high.The two methods results had no significant difference(P0.05)with u test.The quantitative analysis results by TrFIA were respectively 43.13%(0~10 U/L),27.86%(10~100 U/L)and 29.01%(100 U /L).Conclusion TrFIA is a good method of determining HBsAb,especially for the detection of low titer HBsAb.It may distinguish weak immunity and effective immunity for hepatitis B virus in crowd with HBsAb positive by ELISA test.