Comparison of two methods for determination of hepatitis B surface antigen

OBJECTIVE To compare the effectiveness of enzyme-linked immunosorbent assay(ELISA) and time-resolved fluorescence immunoassay(TRFIA) applied for the detection of hepatitis B surface antibody(HBsAb).METHODS A total of 218 samples were detected by each method.Quantitative and qualitative detection of HbsAb was performed in accordance with the National clinical laboratory operating procedures and the instructions requirements supplied by each kit.Results were analyzed and compared.RESULTS The results of 218 serum HBVM tested by TRFIA and ELISA were compared.HBsAg,HBeAg,anti-HBe results of the two methods were in line with the compliance rate,both were more than 90.0%,the difference was not statistically significant.The compliance rates of the results of anti-HBs,anti-HBc were both 88.99%,the differences were statistically significant(P0.05).CONCLUSION TRFIA is superior to the traditional ELISA method in detection of hepatitis B serum markers,which improves the diagnosis rate of hepatitis B.It can also assess changes in patient′s condition and the seriousness of infectious diseases according to the expression of serum markers.It is more accurate and more reliable than the traditional ELISA method,and is worthy of promotion.