ACCELERATIN OF GLUCOCORTICOID TO GLOMERULUS LOSSES IN RATS WITH ADRIAMYCIN NEPHROPATHY

Objective:Whether glucocorticoid exert an acceleration effect or not on glomerulus losses in rats with adriamycin nephropathy was studied.Methods:40 male SD rats were randomly divided into A(control),B(adriamycin),C(adriamycin plus glucocorticoid) and D(glucocorticoid alone)groups.Rats in groups B and C were injected with a single dose of 7mg/kg adriamycin via caudal vein.Urine protein excretion,serum creatinine,serum triglyceride,total cholesterone and renal pathologic changes were observed in 5 rats of every group at the 16th week,the 28th week respectively. Results:Urine protein excretion rate was elevated significantly within one week after injection with adriamycin,and increased to 168.45±76.28mg/d after four weeks. After treatment with glucocorticoid for 12 weeks and 24 weeks(i.e.16 weeks and 28 weeks after injected with adriamycin), the levels of urine protein excretion,serum creatinine,serum triglyceride and total cholesterone in group B were significantly higher than that in group A.Compared with group B, urine protein excretion,serum creatinine,serum triglyceride and total cholesterone in group C were elevated markedly .Parameters from group D varied,urine protein ,serum triglyceride and total cholesterone were higher than group A.In respect of renal pathologic changes scores, group B higher than group A, group C at 16th weeks higher than group B,group D at the both 16th week and 28th week higher than group B.Conclusions: High doses glucocorticoid in long term use can induce glomerlus damage in healthy rats. It can also accelerate glomerulus losses in rats with adriamycin nephropathy.