Effect of 1,25(OH)_2D_3 on HGF expression of rats with adriamycin nephropathy

Objective:To observe the expression of liver cell growth factor(hepatocyte growth factor,HGF) in blood and renal tissue of adriamycin nephrosis rats,the efect of 1,25(OH)2D3 on the HGF expression of minimal change nephrotic syndrome(MCNS),and its protection mechanism.Methods:110 male Wistar rats were randomly divided into normal group(32),and nephropathy group(78 only).Nephropathy group received single tail vein injection of adriamycin in 5mg/kg,building MCNS model,and the normal group was given the same amount of normal saline injection.The urine protein levels of 24 hour urine in all rats were detected on the seventh day of molding: urine protein 30mg/24h for MCNS of successful modeling.Except 5,the other successful modeling rats(73 in total) were randomly divided into two groups: nephropathy group(n=37),and the treatment group(n=36).The 24h urinary protein,serum albumin,cholesterol content of the rats were measured on 7d,14d,28d,42d after molding.At the same time,the level of HGF in blood and renal tissue of rats were detected by using a sandwich ELISA method.Results:When compared with the normal group,HGF expression in blood and renal tissue of rats of control group increased(P0.01).Compared with the control group,the expression level of HGF in the treatment group increased significantly(P0.01).Urinary protein excretion,in comparison with the control group,significantly reduced(P0.01).Conclusion:1,25(OH)2D3 has some protective effect on minimal change nephrotic syndrome.By means of increasing the HGF expression,it can protect the kidney of rats with adriamycin nephropathy.