Establishment of Rapid Propagation System for Lilium regale in vitro

The influence of different concentration hormone(6-BA,NAA,IBA) for the tissue culture of Lilium regale,based on MS medium were studied in order to explore the suitablest level of hormone and to increase the efficiency of the tissue culture of Lilium regale,and design different components of the four medium to find the best medium,look for best growth conditions,What was more,transplanting of Lilium regale was studied to establish the high frequency propagation system of Lilium regale.The following were the experimental results through the observation and analysis of the experiment.The best sterilization method was that shoot tip was soaked in the 75% of alcohol for 30s first,and then put in 0.1% mercuric chloride for 6 minutes,Enrich of MS medium with 6-BA 3 mg/L+NAA 0.2 mg/L+sucrose 30 g+agar 7.5 g is the best.promoted the induction of adventitious shoots and their growth.The optimum rooting medium was MS supplemented with NAA 0.1 mg/L+ IBA 0.01 mg/L.The Fatshedrea lizei needed light in the process of tissue culture.The transplant survival rate was high on the transplant medium of send∶pearlite∶peat=1∶1∶1,survival rate was up to 92%.