Purification and Determination of Polysaccharide from Scutellaria barbata D. Don and Tumor-Inhibitory Effect of Polysaccharide in Mice Bearing of Sarcoma-180

OBJECTIVE To detect the content of purificatus polysaccharide from SPS,and compare the tumor-inhibitory of crude materials and purificated polysaccharide and provide some references for research on chemistry and pharmacology of polysaccharide from cutellaria barbata D.Don.METHODSUV-spectrophotometer and phenol-sulfuric method were used,the detection wavelength was 490 nm and tumor-inhibitory effect of polysaccharide was detected through intracorporeal test.RESULTSThe percent of purificatus polysaccharide was 54.12%,average recovery was 99.66%,the rate of inhibiting tumor of crude and purificatus polysaccharide were 45.96% and 22.14% respectively.CONCLUSIONThis method is simple,convenient,reliable and the anti-tumor activity of crude polysaccharide is better than that of purificatus polysaccharide.