2013•Heilongjiang xumu shouyiRequires access
The effect of vitamin C on the cell cycle and membrane potential of the primary hepatocytes in broilers incubated with copper
Zhaoxin Tang
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Abstract
To investigate the effect of vitamin C on the cell cycle and membrane potential of the primary hepatocytes in broilers incubated with copper,the primary hepatocytes were divided into normal control(groupⅠ) and four stimulus groups treated with 10 μmol/L Cu2+(group Ⅱ),30 μmol/L Cu2+(group Ⅲ),50 μmol/L Cu2+(group Ⅳ),and 50 μmol/L Vc+50 μmol/L Cu2+(group Ⅴ) after changed the medium in 24 h.The cell cycles and cell membrane potentials were measured at 48 h by the methods of flow cytometry(FCM).The result showed that the number of the primary hepatocytes which were treated with 30 μmol/L and 50 μmol/L Cu2+ was significantly increased in stationary phase,and was significantly decreased in proliferative stage,and the cell membrane potential was decreased(P0.05).There was no obvious change in 10 μmol/L Cu2+.The results indicate that vitamin C can significantly inhibit the changes caused by 50 μmol/L Cu2+,it has a protective effect in the cells.