Study on Inhibition of Heat Shock Protein 70 in vitro by Quercetin

Objective To study the HSP70 expression in tumor cells and normal cells treated by different concentrations of quercetin.Methods Treatments of 50,100,150,200 μmol/L quercetin were performed to A549 cells and 2BS cells at 37 ℃ for 6 h,then at 42 ℃ water bath for 1 h.Cells were cultured at 37 ℃ for 2 h recovery.Normal cultured cells were as a normal control.Cells treated by 42 ℃ were as a positive control and cells treated by 0.1% DMSO were as a solvent control.HSP70 levels were detected by western blot assay.Results Along with the increasing quercetin concentrations from 50,100,150 to 200 μmol/L,HSP70 levels decreased.Compared with the group of normal control,there was a significant increase in the group of positive control or solvent control(P0.01).There was no obvious difference between positive control and solvent control(P0.05).For A549 cells,HSP70 levels decreased significantly in the group of 100,150 and 200 μmol/L(P0.05,or P0.01)compared with positive control.There was no significant difference between 150 μmol/L and 200 μmol/L groups(P0.05).For 2BS cells,compared with positive control,HSP70 levels decreased obviously in the group of 50 μmol/L(P0.05)and in the groups of 100,150,and 200 μmol/L(P0.01),but there was no obvious difference between 150 μmol/L and 200 μmol/L groups(P0.05).Conclusions HSP70 expression can be inhibited in A549 or 2BS cells by quercetin,and the effect is the best with 150 μmol/L quercetin treatment.