Establishment of the Cadmium-induced Hepatocyte Damage Model

In this study,the rat primary hepatocyte culture system in vitro was used to study the changes of cell viability in different concentrations of cadmium. In primary hepatocyte cultured at different time points in vitro( 4 h 、 7 h 、 10 h and 24 h), which were incubated with cadmium acetate of different concentrations(0 μmol/L,0.5 μmol/L,1.0 μmol/L,2.0 μmol/L,4.0 μmol/L) for 3 h.The results of MTT assay showed that Cd reduced the motility rat of primary hepatocytes at dose-dependant. After exposed to 4.0 μmol/L cadmium,the cell motility decreased to78.35%,showing a significantly difference from the control group(P0.01).Hepatocytes were cultured for 4 h,7 h and 24 h and treated with 4.0 μmol/L Cd for 3 h in vitro,cell viability was significantly decreased(P0.01). The results suggested that Cd-induced cell viability was at a concentrationdependent manner,and the cell initial state could significantly affect Cd-induced cell viability.