The effect of high copper on the cell cycle and cell membrane potential of primary hepatocyte in broilers

To investigate the effect of high copper on the cell cycle and cell membrance potential of the primary hepatocyte in broiler,the cell were divided into normal control,low copper group(10 μmol/L Cu2+ group),high copperⅠgroup(50 μmol/L Cu2+group)and high copperⅡgroup(100 μmol/L Cu2+ group)after changed the medium in 24h.The cell cycle and cell membrane potential were measured at 48hby the methods of flow cytometry(FCM).The results showed that the G0 / G1 phase was significantly increased(P0.05),and S phase,G2 +M phase were significantly decreased(P0.05)in high copper group.The relative fluorescence intesities(RFI)of cell membrane potential were decreased significantly in high copper group;There was no obvious change in low copper group.In conclusion,high copper could change the cell cycle and cell membrane potential of primary hepatocyte significantly.