Determination of Mycophenolic Acid Concentration in Human Plasma by High Performance Liquid Chromatography

Objective To establish an high performance liquid chromatography method for the determination of mycophenolic acid in human plasma in order to monitor its concentration for pharmacokinetic studies and its concentration in renal transplantation patients.Methods Carbamazepine was used as the internal standard.The content of mycophenolic acid was detected by ultraviolet detector at 250 nm with Diamonsil C18(150 mm×4.6 mm,5 μm)column at a flow rate of 1.2 ml/min.phase A was 0.05 mol/L Na2HPO4(pH=2.88)-acetonitrile(2∶1),while phase B was methol.The mobile phase was phase A while phase B(76∶24).The column temperature was 30℃.Results The linear range was 0.5-50.0 μg/ml(r=0.9996).The average absolute recovery was 91.4%,100.6%,88.6% and the method recovery was 107.3%,108.9%,106.6% for the high,middle,low control samples.The relative standard deviation obtained for inter-and intra-assay precision was both less than 9%.Conclusion This method is user-friendly,sensitive,reproducible,and suitable for the determination of mycophenolic acid in human plasma for pharmacokinetics and for monitoring mycophenolic acid concentration in patients.