Determination of mycophenolic acid in plasma by HPLC

OBJECTIVE To establish a method for determination of mycophenolic acid (MPA) in plasma by HPLC.METHODS A supelcosil TM LC 18 column(5 μm, 4.6 mm×150 mm)was adopted. Mobile phase was a mixture of methanol and 0.025 mol·L -1 NaH 2PO 4 (containing 1% acetic acid, pH 5.0 )(45∶55) .The temperature was set at 25℃ and the flow rate was 1.2 mL·min -1 . The UV detector was operated at 218 nm.RESULTS The assay procedure was shown to produce linear calibration curves over the range of 0.25 to 50 mg·L -1 of MPA in plasma (r= 0.9998 ). Within the range, the recovery rate was ( 102.4 ± 5.8 )%. And the relative standard deviation (RSD) within a day and between days were less than 7.0% and 7.9% , respectively. CONCLUSIONS This method is found to be reproducible, convenient and sensitive for determination of plasma concentration and clinical pharmacokinetic study of mycophenolic acid.