Quantification of mycophenolic acid in human plasma by high performance liquid chromatography

OBJECTIVE To establish an high performance liquid chromatography(HPLC) quantitative method for mycophenolic acid(MPA) in human plasma.METHODS HPLC analysis was performed on that chromatographic column was Agilent C18(150.0mm×4.6mm,5μm),the mobile phase was 0.05mol/L Na2HPO4(pH2.88)-acetonitrile(65∶35),the flow rate was 1.0mL/min,the column tempratrue was 35℃,and the detection wavelength was 240nm.The carbamazepine was internal standard.RESULTS The ratio of blood medicine mass concentration and peak area was good linear relationship(r=0.9997) as the blood medicine mass concentration range of MPA was in 0.5 ~ 50.0mg/L.The average extracting recovery rate was 89.77%,99.43%,100.39% and method recovery was 102.33%,104.62%,107.39% in the low,middle and high control samples,respectively.The relative standard deviation obtained from intraday and by day assay precisions was both less than 7%.The sample at room temperature for 24h,-20℃ freezing and freeze thawing had good stability.CONCLUSION The method is simple,sensitive,high recovery and good reproducibility,suitable for monitoring the blood medicine mass concentration of MPA and studying its pharmacokinetics in human plasma.