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2004•Analytical LaboratoryRequires access

Determination of rutin by inhibitory kinetic spectrophotometry

Zhang Ai-mei

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Abstract

An inhibitory kinetic spectrophotometric method for the determination of rutin was proposed. The method was based on the inhibition of rutin to the discoloring reaction of acid bordeaux R oxidized by potassium periodate solutions. The optimum conditions and kinetic parameters were studied in detail. The linear range was 0.02～0.4 μg/mL with the detection limit of 5.0×10~(-9) g/mL. The method was applied to determine the trace rutin in sophorine and pharmacertical preparations.

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What this paper is about

An inhibitory kinetic spectrophotometric method for the determination of rutin was proposed. The method was based on the inhibition of rutin to the discoloring reaction of acid bordeaux R oxidized by potassium periodate solutions. The optimum conditions and kinetic parameters were studied in detail. The linear range was 0.02～0.4 μg/mL with the detection limit of 5.0×10~(-9) g/mL. The method was applied to determine the trace rutin in sophorine and pharmacertical preparations.

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Available abstract

An inhibitory kinetic spectrophotometric method for the determination of rutin was proposed. The method was based on the inhibition of rutin to the discoloring reaction of acid bordeaux R oxidized by potassium periodate solutions. The optimum conditions and kinetic parameters were studied in detail. The linear range was 0.02～0.4 μg/mL with the detection limit of 5.0×10~(-9) g/mL. The method was applied to determine the trace rutin in sophorine and pharmacertical preparations.

Key concepts: Rutin, Chemistry, Potassium periodate, Detection limit, Spectrophotometry, Chromatography, Kinetic energy, Linear range

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