Study on the Determination of Rutin by Kinetic Spectrophotometry
Daojie Liu
Abstract
Daojie Liu
Abstract
A catalytic kinetic spectrophotometric method for the determination of rutin was proposed. The method was based on the catalysis of rutin to the discoloring reaction of rhodamine 6G oxidized by potassium peridate solutions. The optimum conditions and kinetic parameters were studied in detail. The linear range was 0.02~1.5 μg/mL. The detection limit was 1.0×10~(-8) g/mL. The method has been applied to the determination of trace rutin in pharmacertical preparation.
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A catalytic kinetic spectrophotometric method for the determination of rutin was proposed. The method was based on the catalysis of rutin to the discoloring reaction of rhodamine 6G oxidized by potassium peridate solutions. The optimum conditions and kinetic parameters were studied in detail. The linear range was 0.02~1.5 μg/mL. The detection limit was 1.0×10~(-8) g/mL. The method has been applied to the determination of trace rutin in pharmacertical preparation.
Key concepts: Rutin, Chemistry, Detection limit, Rhodamine 6G, Spectrophotometry, Linear range, Catalysis, Kinetic energy