Kinetic Spectrophotometric Determination of Rutin by Its Inhibitory Effect on the Oxidation of Amaranth by Potassium Periodate
Shuhao Wang, Lingyun Du, Xingjun Yao, Xueli Niu, Huisheng Zhuang
Abstract
Shuhao Wang, Lingyun Du, Xingjun Yao, Xueli Niu, Huisheng Zhuang
Abstract
A novel kinetic spectrophotometric method is described for the determination of rutin. The method is based on the inhibitory effect of rutin on the oxidation reaction of amaranth by potassium periodate in acidic media at 100 degrees C. The linear range for the determination of rutin is 0.02 - 0.50 microg/ml, and the detection limit is 0.014 microg/ml. The method has been successfully applied to the determination of rutin in medicine of rutin tablet and traditional Chinese medicine.
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A novel kinetic spectrophotometric method is described for the determination of rutin. The method is based on the inhibitory effect of rutin on the oxidation reaction of amaranth by potassium periodate in acidic media at 100 degrees C. The linear range for the determination of rutin is 0.02 - 0.50 microg/ml, and the detection limit is 0.014 microg/ml. The method has been successfully applied to the determination of rutin in medicine of rutin tablet and traditional Chinese medicine.
Key concepts: Rutin, Amaranth, Chemistry, Potassium periodate, Detection limit, Periodate, Potassium, Chromatography