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Simultaneous determination of four components in Rhododendron athopogoides by HPLC

Jing Li

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Abstract

OBJECTIVE To establish a quantitative method for simultaneous determination of rutin,hyperoside,quercetin,kaempferol in Rhododendron athopogoides by RP-HPLC.METHODS The sample was separated on column of Hypersil ODS-2(250 mm×4.6 mm,5 μm)with gradient elution,the mobile phase was comprised of methanol and water(0.04% phosphoric acid).The flow rate was 1mL·min-1.The detection wavelength was at 360 nm and the column temperature was 30 ℃.RESULTS The linear ranges of rutin,hyperoside,quercetin and kaempferol were 0.042-5.95 μg(r=0.9999),0.490-2.450 μg(r=0.9997),0.008-1.600 μg(r=0.9998),0.014-0.142 μg(r=0.9999),respectively.The average recovery were 99.13%(RSD=0.56%),98.95%(RSD=0.29%),100.04%(RSD=1.72%),97.27%(RSD=0.65%),respectively.CONCLUSION The method is rapid and precise.

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OBJECTIVE To establish a quantitative method for simultaneous determination of rutin,hyperoside,quercetin,kaempferol in Rhododendron athopogoides by RP-HPLC.METHODS The sample was separated on column of Hypersil ODS-2(250 mm×4.6 mm,5 μm)with gradient elution,the mobile phase was comprised of methanol and water(0.04% phosphoric acid).The flow rate was 1mL·min-1.The detection wavelength was at 360 nm and the column temperature was 30 ℃.RESULTS The linear ranges of rutin,hyperoside,quercetin and kaempferol were 0.042-5.95 μg(r=0.9999),0.490-2.450 μg(r=0.9997),0.008-1.600 μg(r=0.9998),0.014-0.142 μg(r=0.9999),respectively.The average recovery were 99.13%(RSD=0.56%),98.95%(RSD=0.29%),100.04%(RSD=1.72%),97.27%(RSD=0.65%),respectively.CONCLUSION The method is rapid and precise.

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Available abstract

OBJECTIVE To establish a quantitative method for simultaneous determination of rutin,hyperoside,quercetin,kaempferol in Rhododendron athopogoides by RP-HPLC.METHODS The sample was separated on column of Hypersil ODS-2(250 mm×4.6 mm,5 μm)with gradient elution,the mobile phase was comprised of methanol and water(0.04% phosphoric acid).The flow rate was 1mL·min-1.The detection wavelength was at 360 nm and the column temperature was 30 ℃.RESULTS The linear ranges of rutin,hyperoside,quercetin and kaempferol were 0.042-5.95 μg(r=0.9999),0.490-2.450 μg(r=0.9997),0.008-1.600 μg(r=0.9998),0.014-0.142 μg(r=0.9999),respectively.The average recovery were 99.13%(RSD=0.56%),98.95%(RSD=0.29%),100.04%(RSD=1.72%),97.27%(RSD=0.65%),respectively.CONCLUSION The method is rapid and precise.

Key concepts: Chemistry, Hyperoside, Rutin, Chromatography, Kaempferol, Phosphoric acid, Quercetin, Gradient elution

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